Diagnostics Large Animal Surgery Supplemental Notes: Revision history

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27 October 2025

  • curprev 01:1701:17, 27 October 2025‎ PedroJooMiguelNo talk contribs‎ 5,818 bytes +5,818‎ Created page with "Oligo 1 carries a fluorescein label at its 3’ finish, whereas oligo 2 carries another label (LC Pink 640) at its 5’ finish. The sequences of the 2 oligonucleotides are chosen in order that they hybridize to the amplified DNA fragment in a head-to-tail arrangement. When the oligonucleotides hybridize on this orientation, the two fluorescence dyes are positioned in shut proximity to one another. The first dye (fluorescein) is excited by the LightCycler’s LED (light e..."
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